Nine HS2 security staff were injured when 30 people attacked them on the A413 - with passing traffic "narrowly" missing people in the road.
Around 30 people attacked personnel from the HS2 construction site in Small Dean, Wendover, on Friday night.
Police were called at around 8.30pm on March 26.
Nine members of security staff were apparently injured and property damaged.
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Much of what police are calling a "violent disorder" happened on the A413, with passing traffic narrowly missing people in the road.
Thames Valley Police say this was a "serious" incident, but fortunately no one was badly hurt.
A spokesman for Thames Valley Police said: “This was an unacceptable outbreak of violent disorder where a number of people were attacked in what appears to have been a pre-meditated and orchestrated incident.
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“This incident took place in close proximity to the A413, and although thankfully no one was seriously hurt, this was a serious incident.
“We are appealing to anybody who was driving in the area at around the time and who has dash-cam in their vehicles to please check this and contact the police if it has captured anything that could assist this investigation.
“You can call 101, quoting incident reference 2343 of 26 March or make a report online.
“You can also report 100 per cent anonymously via the independent charity Crimestoppers on 0800 555 111.”
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